自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

您目前所在的位置:首页 - 期刊简介 - 详细页面

中南大学学报(英文版)

Journal of Central South University

Vol. 23    No. 2    February 2016

[PDF Download]    [Flash Online]

    

Simultaneous separation and determination of four main isoflavonoids in Astragali Radix by an isocratic LC/ESI-MS method
WANG Yu-ling(王玉林)1, 2, LIANG Yi-zeng(梁逸曾)2, ZHANG Jie(张洁)2, FENG Xiao-liang(冯晓亮)1, GE Cheng-sheng(葛承胜)1, HUANG Lan-fang(黄兰芳)2

1. Department of Chemical and Materials Engineering, Quzhou College, Quzhou 324000, China;
2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Abstract:A simple, reliable and rapid isocratic liquid chromatography (LC)-mass spectrometric detection (MS) coupled with electrospray ionization (ESI) method for simultaneous separation and determination of calycosin-7-O-β-D-glucoside, ononin, calycosin and formonometin in Astragali Radix was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using water and acetonitrile (70:30, v/v) containing 0.2% (v/v) acetic acid as a mobile phase and a 2.0 mm×150 mm Hypersil-Keystone C18 column. Selective ion monitoring (SIM) mode and [M+H]+ ions at m/z 447, 431, 285 and 269 were used for quantitative analysis of four main active components above mentioned. The calibration curves were linear in the range of 0.4-175.0 μg/ml for calycosin-7-O-β-D-glucoside, 0.2-146.0 μg/ml for ononin, 0.4-210.0 μg/ml for calycosin and 0.5-217.0 μg/ml for formonetion, respectively. The limits of quantification (LOQ) and detection (LOD) were 0.4 μg/ml and 0.08 μg/ml for calycosin-7-O-β-D-glucoside, 0.2 μg/ml and 0.06 μg/ml for ononin, 0.4 μg/ml and 0.1 μg/ml for calycosin, 0.5 μg/ml and 0.1 μg/ml formonetion, respectively. The standard recoveries were in the range of 96.5%-104.7%. The developed method has successfully been used for the determination of four main flavonoids in Astragali Radix from various sources and can be used for identification, differentiation and quality evaluation of Astragali Radix.

 

Key words: liquid chromatography; electrospray ionization (ESI); mass spectrometric detection (MS); isoflavonoids; Astragali Radix

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
版权所有:《中南大学学报(自然科学版、英文版)》编辑部
地 址:湖南省长沙市中南大学 邮编: 410083
电 话: 0731-88879765(中) 88836963(英) 传真: 0731-88877727
电子邮箱:zngdxb@csu.edu.cn 湘ICP备09001153号