自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 14    No. 4    August 2007

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Isolation of Leptospirillum ferriphilum by single-layered solid medium
LIU Jian-she(柳建设)1,2, XIE Xue-hui(谢学辉)1, XIAO Sheng-mu(肖升木)1,WANG Xiu-mei(王秀美)1, ZHAO Wen-jie(赵文杰)1, TIAN Zhuo-li(田卓力)1

1. School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China;2. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China

Abstract:According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope(SEM) and transmission electron microscope (TEM). The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum. Additionally, physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 ℃, respectively. The culture of it is used to leach a complex concentrate chalcopyrite, the leaching efficiencies of copper and iron are 1.93 % and 13.74 %, respectively, and it is more effective than the A.ferrooxidans culture in the leaching of the complex concentrate chalcopyrite.

 

Key words: Leptospirillum ferriphilum; isolation; purification; solid medium; bioleaching; concentrate chalcopyrite

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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