自然科学版 英文版
自然科学版 英文版
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中南大学学报(英文版)

Journal of Central South University

Vol. 14    No. 4    August 2007

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Bioleaching of chalcopyrite by mixed culture of moderately thermophilic microorganisms
WU Chang-bin(邬长斌)1,2, ZENG Wei-min(曾伟民)2, ZHOU Hong-bo(周洪波)2,3, FU Bo(符 波)2,HUANG Ju-fang(黄菊芳)3, QIU Guan-zhou(邱冠周)2,3, WANG Dian-zuo(王淀佐)3

1. China Ocean Mineral Resources Research and Development Association, Beijing 100860, China;2. School of Resource Processing and Bioengineering, Central South University, Changsha 410083, China;3. Key Laboratory of Biometallurgy, Ministry of Education, Changsha 410083, China

Abstract:A mixed culture of moderately thermophilic microorganisms was enriched from acid mine drainages(AMDs) samples collected from several sulphide mines in China, and the bioleaching of chalcopyrite was conducted both in shake flask and bioreactor. The results show that in the shake flask, the mixture can tolerate 50 g/L chalcopyrite after being acclimated to gradually increased concentrations of chalcopyrite. The copper extraction increases obviously in bioleaching of chalcopyrite with moderately thermophilic microorganisms supplemented with 0.4 g/L yeast extract at 180 r/min, 74% copper can be extracted in the pulp of 50 g/L chalcopyrite after 20 d. Compared with copper extractions of mesophilic culture, unacclimated culture and acclimated culture without addition of yeast extract, that of accliniated culture with addition of yeast extract is increased by 53%, 44% and 16%, respectively. In a completely stirred tank reactor, the mass fraction of copper and total iron extraction reach up to 81% and 56%, respectively. The results also indicate that it is necessary to add a large amount of acid to the pulp to extract copper from chalcopyrite effectively.

 

Key words: bioleaching; moderately thermophilic microorganisms; acclimation; chalcopyrite; shake flask experiment; stirred tank reactor

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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